Solution Information | help | |
Enzyme: | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase | |
inhibitor: | BDBM85279 | |
substrate: | n/a | |
Solution Type: | Aqueous | |
pH at Preparation: | 8.0 | |
Temp. Prep.: | 37 | |
Comments: | MurE | |
pH Buffer 1: | MgCl2 | |
Concentration: | 15 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 2: | Hepes | |
Concentration: | 50 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 3: | ATP | |
Concentration: | 700 uM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 4: | Triton X-114 | |
Concentration: | 0.005% | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 5: | UDP-Mur-NAc-L-Ala-D-Glu | |
Concentration: | 100 uM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 6: | L-Lys | |
Concentration: | 1.5 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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